Seeks a Flow Cytometry Scientist for its Immune Monitoring and Discovery Platform (IMDP)

The Pelotonia Institute for Immuno-Oncology (PIIO) at OSUCCC - James

Seeks a Flow Cytometry Scientist for its Immune Monitoring and Discovery Platform (IMDP)

The PIIO Immune Monitoring and Discovery Platform (IMDP) at The Ohio State University (OSU) is seeking an innovative flow cytometry Scientist. The IMDP delivers cutting-edge technology to analyze the immune system's response to cancer immunotherapy. IMDP flow cytometry resources include Cytek Aurora Spectral Cell Sorter, Cytek Aurora Spectral Analyzer, Cytek Northern Lights, and BD FACSMelody.

The successful candidate will:

• Understand the operation of flow analysis and cell sorting devices in depth.
• Apply a comprehensive knowledge of immunology systems to support immuno-oncology (IO) investigators in achieving the best outcomes and understanding their findings.
• Possess the necessary education and/or experience to work autonomously, act as a resource for users, train newcomers, and assist self-operators with instrument issues.
• Have at least two years of experience with one or more of the following instrument systems: Cytek Aurora Spectral Cell Sorter, Cytek Aurora Spectral Analyzer, Cytek Northern Lights, and BD FACSMelody (experience with other instruments may also be acceptable (e.g. BD Influx, BD FACS Aria/Fusion/S6, BD Symphony, ACEA Novocyte).

Preference will be given to applicants (1) with at least 5-10 years of experience in flow cytometry, (2) extensive experience with cell sorting and (3) proficiency in FlowJo and Omiq. Applicants with less years of experience will be considered if they have substantive knowledge in spectral flow cytometry.

Job Duties include:

• Instrument maintenance, standardization, and quality assurance:
• Establish and/or modifies and/or implements Standard Operating Procedures for routine instrument maintenance and performance evaluation.
• Perform instrument setup and acquisition of samples independently.
• Perform calibration/alignment, maintenance, trouble-shooting, repair of cytometers and associated facility instruments.
• Document instrument performance, maintenance, service, and corrective actions.
• Cytometry training:
• Provide training for technicians and investigators with respect to the basics of the technology, instrument startup/shutdown and maintenance, data acquisition and transfer, and data analysis as needed.
• Experimental design and optimization:
• Work together with the laboratory Director to advise investigators regarding experimental design and data analysis.
• Educate core laboratory clients with respect to sample requirements for specific experimental goals.
• Work with investigators to explore and optimize current and emerging techniques.
• Core management:
• Assess inventory and order supplies when needed.
• Conduct operations of the facility within safety guidelines for instrument operation, as well as chemical and biological safety standards
• Maintain reporting and billing, process costing and fee development, budget development and management.
• Manage technical staff including training, tasking, goal-setting, development, and mentoring.
• Continuing education:
• Maintain technical currency, identify, and assess developing technologies, platforms, reagents, and methods. Import and deploy as appropriate.
• Present novel assays and techniques to research group.
• Assay development:
• Work independently or together with other laboratory staff to design and optimize new analytical methods.
• Work with Director to evaluate new and existing methods with respect to accuracy, precision, and reproducibility.
• Data analysis:
• Work independently or together with Director to establish analysis strategies for flow cytometry-based assays.
• Evaluate new tools for data analysis and data reduction. Train and implement others as required.

Education:

• Bachelor's Degree OR Master's Degree in Biological sciences or related discipline

Experience (Training and Coursework):

• Proficient knowledge of immunology, genetics, and flow cytometry.
• Demonstrated success training and supervising others.
• Demonstrated experience analyzing data from flow cytometry platforms using specialized analysis software and reducing that data to figures suitable for scientific presentations (FlowJo, DiVa and Microsoft Office products (Word, Excel, PowerPoint) and other common tools).
• Five (5) years of related experience, to include supervisory/management experience.
• Specialized training (theoretical and practical) in the application and conduct of flow cytometry practices.
• Training in bacteriological, viral, chemical, and physical safety.
• Proficiency with Macintosh and PC computers and handheld devices.
• Able to work safely in a BSL2 environment.
• Able to safely work with lasers and high voltage.
• Able to work independently.

To apply: Send cover letter, CV, contact information for three references via email to James Fant at James.Fant@osumc.edu.

About Us:
The PIIO is a comprehensive bench-to-bedside research initiative focused on harnessing the body's immune system to fight cancer at all levels (https://cancer.osu.edu/piio). Launched in July 2019 as part of a $102 Million pledge from Pelotonia (pelotonia.org), the PIIO represents Ohio State's commitment to grow the exciting area of immuno-oncology. Led by founding director and renowned immunologist Zihai Li, MD, PhD, the PIIO builds upon a rich portfolio of active investigator-initiated IO clinical trials being conducted at the OSUCCC - James.

Research in the PIIO centers on Systems IO (a systems-wide strategy to study the anti-cancer immune response holistically to discover the next IO breakthroughs and create effective cancer immunotherapies) and Translational IO (where we turn discoveries into new or improved cancer treatments and broaden the indications and patient populations that can be treated with cellular therapy). Our Immune Monitoring and Discovery Platform (IMDP) supports each center, providing comprehensive cell-based and molecule-based immunoassay services to support basic, translational, and clinical IO studies. The PIIO's Immuno-Oncology Informatics Group (IOIG) provides data analytics for flow and mass cytometry, bulk and single-cell RNA-Seq, spatial transcriptomics and imaging, neoantigen discovery, and more.

The OSUCCC - James is a National Cancer Institute (NCI)-designated comprehensive cancer center, rated "Exceptional," the highest rating given to cancer centers by the NCI; is one of only a few centers funded by the NCI to conduct phase I, II and III clinical trials on novel anticancer drugs; and includes the third-largest freestanding cancer hospital in the country. Ohio State is located in Columbus-a highly ranked, modern, affordable, friendly and diverse city. The Ohio State University is an EOE/AA/M/F/D/V employer.